Date Published:

2018 Mar 02

Abstract:

Atopic dermatitis is an allergic inflammatory skin disease characterized by the production of the type 2 cytokines in the skin by type 2 innate lymphoid cells (ILC2s) and T helper 2 (T2) cells, and tissue eosinophilia. Using two distinct mouse models of atopic dermatitis, we show that expression of retinoid-related orphan receptor α (RORα) in skin-resident T regulatory cells (T) is important for restraining allergic skin inflammation. In both models, targeted deletion of RORα in mouse T led to exaggerated eosinophilia driven by interleukin-5 (IL-5) production by ILC2s and T2 cells. Expression of RORα in skin-resident T suppressed IL-4 expression and enhanced expression of death receptor 3 (DR3), which is the receptor for tumor necrosis factor (TNF) family cytokine, TNF ligand-related molecule 1 (TL1A), which promotes T functions. DR3 is expressed on both ILC2s and skin-resident T Upon deletion of RORα in skin-resident T, we found that T were no longer able to sequester TL1A, resulting in enhanced ILC2 activation. We also documented higher expression of RORα in skin-resident T than in peripheral blood circulating T in humans, suggesting that RORα and the TL1A-DR3 circuit could be therapeutically targeted in atopic dermatitis.