@article {1490768,
	title = {Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis},
	journal = {Immunity},
	volume = {49},
	number = {6},
	year = {2018},
	month = {2018 12 18},
	pages = {1062-1076.e6},
	abstract = {Neutrophils require directional cues to navigate through the complex structure of venular walls and into inflamed tissues. Here we applied confocal intravital microscopy to analyze neutrophil emigration in cytokine-stimulated mouse cremaster muscles. We identified differential and non-redundant roles for the chemokines CXCL1 and CXCL2, governed by their distinct cellular sources. CXCL1 was produced mainly by TNF-stimulated endothelial cells (ECs) and pericytes and supported luminal and sub-EC neutrophil crawling. Conversely, neutrophils were the main producers of CXCL2, and this chemokine was critical for correct breaching of endothelial junctions. This pro-migratory activity of CXCL2 depended on the atypical chemokine receptor 1 (ACKR1), which is enriched within endothelial junctions. Transmigrating neutrophils promoted a self-guided migration response through EC junctions, creating a junctional chemokine "depot" in the form of ACKR1-presented CXCL2 that enabled efficient unidirectional luminal-to-abluminal migration. Thus, CXCL1 and CXCL2 act in a sequential manner to guide neutrophils through venular walls as governed by their distinct cellular sources.},
	keywords = {Abdominal Muscles, Animals, Chemokine CXCL1, Chemokine CXCL2, Duffy Blood-Group System, Endothelial Cells, Gene Expression Profiling, Gene Expression Regulation, Intercellular Junctions, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Neutrophils, Receptors, Cell Surface, Transendothelial and Transepithelial Migration, Tumor Necrosis Factor-alpha},
	issn = {1097-4180},
	doi = {10.1016/j.immuni.2018.09.018},
	author = {Girbl, Tamara and Lenn, Tchern and Perez, Lorena and Rolas, Lo{\"\i}c and Barkaway, Anna and Thiriot, Aude and Del Fresno, Carlos and Lynam, Eleanor and Hub, Elin and Thelen, Marcus and Graham, Gerard and Alon, Ronen and Sancho, David and von Andrian, Ulrich H and Voisin, Mathieu-Benoit and Rot, Antal and Nourshargh, Sussan}
}
