von Andrian Lab Videos
 

 

NOCICEPTIVE SENSORY NEURONS DRIVE INTERLEUKIN-23 MEDIATED PSORIASIFORM SKIN INFLAMMATION


CD11c-YFP+ dermal dendritic cells (DDC) show diverse interactions with NaV1.8+ nociceptor

Nature Supplementary Movie s1.mp4

NaV1.8-Cre-TdTomato mice in which nociceptors express fluorescent TdTomato protein (red) were reconstituted with CD11c-YFP bone marrow to visualize interactions of DDC (green) with cutaneous nerves in intact ears of anesthetized mice. Videos are displayed as maximum intensity projections of time-lapse recordings of 3D image stacks generated on a multi-photon intravital microscope (Prairie Technologies) using a 20X water immersion objective (Nikon). Scale bar = 40 µm. Playback is accelerated 300x over real-time.

 


MHC-II-GFP+ dendritic cells migrate along GFAP+ Schwann cells in mouse skin

Nature Supplementary Movie s2.mov

Glial fibrillary acidic protein (GFAP)-Cre-TdTomato mice in which Schwann cells express the fluorescent TdTomato protein (red) were reconstituted with MHC-II-GFP bone marrow to visualize MHC-IIbright dermal dendritic cell interactions (green) with cutaneous nerves in intact ears of anesthetized mice. Videos are displayed as maximum intensity projections of time-lapse recordings of 3D image stacks generated on a multi-photon intravital microscope (Prairie Technologies) using a 20X water immersion objective (Nikon). Scale bar = 10 µm. Playback is accelerated 300x over real-time.

 

 



For details on experimental methods & techniques see:
Riol-Blanco, L., Ordovas-Montanes, J., Perro, M., Naval, E., Thiriot, A., Alvarez, D., Paust, S., Wood, J.H., and von Andrian, U.H. Nociceptive sensory neurons drive interleukin-23 mediated psoriasiform skin inflammation. Nature, ePub 2014, April 23.[Full Text PDF] [Supplementary Material]