von Andrian Lab Videos
 

 

A NOVEL ENDOTHELIAL L-SELECTIN LIGAND ACTIVITY IN LYMPH NODE MEDULLA THAT IS REGULATED BY α(1,3)-FUCOSYLTRANSFERASE-IV

JEM Supplementary Video 1

Topography of MECA-79-reactive L-selectin ligands in PLNs.


L1-2 cells that do not express L-selectin do not interact with venular endothelium, although some of these relatively large cells become mechanically retained in capillaries.


JEM Supplementary Video 3

In the absence of antibody, L1-2 (L-selectin) transfectants roll in all venular orders, but not in arterioles.


JEM Supplementary Video 4

After the recipient mouse was injected with 10 mg/kg MECA-79, L1-2 (L-selectin) cells fail to roll in high order venules, but several do roll in order I and II venules.


Animated 3-D reconstruction of serial confocal sections through a subiliac LNs 24 h after treatment with mAb MECA-79 and adoptive transfer of green fluorescent T cells.


For details on experimental methods & techniques see:
M'Rini, C., Cheng, G., Schweitzer, C., Cavanagh, L.L., Palframan, R.T., Mempel, T.R., Warnock, R.A., Lowe, J.B., Quackenbush, E.J., von Andrian, U.H. A Novel Endothelial L-Selectin Ligand Activitiy in Lymph Node Medulla that is Regulated by α(1,3)-Fucosyltransferase-IV. Journal of Experimental Medicine, 198 (9): 1301-1312, 2003.[Full Text PDF]