von Andrian Lab Videos
 

 

THE S1P-ANALOG FTY720 DIFFERENTLY MODULATES T CELL HOMING VIA HEV: T CELL-EXPRESSED S1P1 AMPLIFIES INTIGRIN ACTIVATION IN PERIPHERAL LYMPH NODES BUT NOT IN PEYER'S PATCHES

Video S1. S1P1 does not affect cell motility within the popliteal LNs (3.31 MB)

CMFDA-labeled S1P1+/ (green) and CMTMR-labeled S1P1-/-CD4+ SP T cells (red) were adoptively transferred into WT mice and their migratory behavior analyzed after 24 hours./p>

 



For details on experimental methods & techniques see:
Halin, C., Scimone, M.L., Bonasio, R., Gauguet, J-M., Mempel, T.R., Quackenbush, E., Proira, R.L., Mandala, S., and von Andrian, U.H. The S1P-analog FTY720 differently modulates T-cell homing via HEV: T-cell-expresses S1P1 ampliphies integrin activation in peripheral lymph odes but not in Peyer patches. Blood, 106(4): 1314-1322, 2005. [Full Text PDF]